Specific nuclease are used for genome editing, and are broadly divided into artificial nucleases and RNA-guided nucleases. An artificial restriction enzyme is an artificial restriction enzyme that combines a DNA-binding domain designed and produced to specifically bind to a target sequence with the DNA-cleaving domain (nuclease domain) of the restriction enzyme FokI. When a pair of artificial nucleases bind to adjacent target sequences, they form dimers in the nuclease domains and induce DNA double-strand breaks (DSBs) in the spacer sequences between the target sequences. On the other hand, in the RNA-induced type, a short-stranded guide RNA binds to the target, It seems to be a system that induces DSB by CRISPR-associated 9 with two nuclease domains.

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